DNA Vectors
New England Biolabs, Inc. pSNAP-tag (T7)-2 Vector – 20 µg
pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl chloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
Eurofins Discoverx PH PROLABEL DETECTN KIT 800DP
The PathHunter® ProLabel Detection Kit is for use with PathHunter Cell Lines expressing ProLabel® (PL) or ProLink™ (PK) expression or cloning vectors. The kit measures the total amount of ProLabel- or ProLink-tagged protein expressed in cells. Functional assays using PathHunter Cell Lines expressing both a ProLabel- or ProLinktagged protein and EA require the PathHunter Detection Kit (93-0001). The assay can be used in both 96-well and 384-well microplate formats. 800 dp (384-well)
IBA LifeSciences StrGate Acptr Vctr pCSG-IBA104
pCSG-IBA104 is a large expression vector with universal features for transient expression of target proteins with an N-terminal Twin-Strep-tag as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide.
IBA LifeSciences pASK-IBA6C
pASK-IBA6C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, and the sequence for a protease cleavage site.Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tagII can be removed by the protease factor Xa.
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt1
The pASG-IBAwt1 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt2
The pASG-IBAwt2 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via Entry Vector is possible.
New England Biolabs, Inc. M13mp18 RF I DNA – 10 µg
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the -Galactosidase gene. When a fragment of DNA is inserted into one of these sites, the -Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate.
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
New England Biolabs, Inc. M13K07 Helper Phage – 1.8 ml
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
Sigma Aldrich Fine Chemicals Biosciences CAS9 Blasticidin Lenti Plasmid, 50 uL vial
This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Functional Genomics/Screening /Target Validation/Genome Editing.